Plückthun

Explain what you did in this thesis/project (IMPORTANT: provide a general overview without going into details, for example: measuring protein activity, designing a binding pocket, developing a purification protocol, etc.):

In silico design of a [protein] binding pocket using Rosetta, Protein expression and purification, measuring binding affinity and specificity (by fluorescence anisotropy) of the binding pocket towards target and non-target

Which techniques did you work with during your course (for example: Cryo-EM, Cloning, PCR, NMR, activity assays, protein purification, etc.)?

Rosetta computational de novo design, PCR & Ligation, plasmid transfromation and sequencing, protein purification, KD determination by fluorescence anisotropy on a microplate reader

What programs did you work with? (for example: excel, prism, R, etc.):

Rosetta, alpha fold, prism, excel

Name the two most used techniques/programs out of the ones you worked with:

PCR, Fluorescence anisotropy

State what you spent most time with (for example: data analysis, programming, troubleshooting, cloning, sequencing, etc):

Computational pocket design

On a scale from 1-5: How supervised were you during your work? (5 being completely supervised, 1 being totally free in every decision):

3

What was the final product of your lab work?

Thesis