Plückthun Bachelor reports

What was the main aim of the project: 

• -

• In silico design of a [protein] binding pocket using Rosetta, Protein expression and purification, measuring binding affinity and specificity (by fluorescence anisotropy) of the binding pocket towards target and non-target

Which techniques were used:

• Fluorescence Anisotropy, protein purification

• Rosetta computational de novo design, PCR & Ligation, plasmid transfromation and sequencing, protein purification, KD determination by fluorescence anisotropy on a microplate reader

Which programs were used:

• Python, Rosetta

• Rosetta, alpha fold, prism, excel

Which two of these techniques or programs were used the most:

• Nickel columns, Dialysis

• PCR, Fluorescence anisotropy

What did you spend most of your time on:

• -

• Computational pocket design

How supervised was your work: (5 being completely supervised, 1 being totally free in every decision)

• 5/5

• 3/5

What was the final product of the lab work:

• Report, Thesis

• Thesis

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